ABC3735 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:1,000 ELISA: 1:1,000 - 1:2,500
Reactivity	Bacillus species
Host	Goat
Clonality	Polyclonal
Immunogen	Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.
Specificity	Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species].  Cross reactivity against Uricase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID Q5WBJ3
Background	Anti-Uricase antibody detects uricase. Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localized in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form; and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation. Anti-Uricase antibody is ideal for investigators involved in Cell Signaling and Signal Transduction research.
Synonyms	goat anti-uricase antibody, HRP conjugated goat anti-uricase antibody, Urate oxidase antibody
Note	Anti-Uricase antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.
Reference Data

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