BLRO_MYRVE Goat Polyclonal Antibody

CAT#: TA396669S

Bilirubin Oxidase Antibody Peroxidase Conjugated



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CNY 2,119.00


货期*
4周

规格
    • 25 ul

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Specifications

Product Data
Applications ELISA, IP, WB
Recommend Dilution WB: 1:500 - 1:2,500
ELISA: 1:1,000 - 1:5,000
Reactivity Myrothecium verrucaria
Host Goat
Clonality Polyclonal
Immunogen Bilirubin Oxidase [Myrothecium verrucaria]
Specificity Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].  Cross reactivity against Bilirubin Oxidase from other sources is unknown.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.0 mg/mL - lot specific
Conjugation HRP
Storage Condition Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background Bilirubin Oxidase is key in the oxidation of bilirubin and other tetrapyrroles. This enzyme belongs to the family of oxidoreductases, to be specific those acting on the CH-CH group of donor with oxygen as acceptor. The systematic name of this enzyme class is bilirubin:oxygen oxidoreductase. This enzyme is also called bilirubin oxidase M-1. This enzyme participates in porphyrin and chlorophyll metabolism.
Synonyms goat anti-Bilirubin Oxidase HRP conjugation, goat anti-Bilirubin Oxidase Antibody Peroxidase Conjugated
Note Anti-Bilirubin Oxidase has been tested by western blot and is suitable to be assayed against 1.0 ug of Bilirubin Oxidase [Myrothecium verrucaria] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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