NAM-DH Goat Polyclonal Antibody

CAT#: TA396672S

N-Acylmannosamine-1-Dehydrogenase Antibody Peroxidase Conjugated



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CNY 2,119.00


货期*
4周

规格
    • 25 ul

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Specifications

Product Data
Applications ELISA, IP, WB
Recommend Dilution WB: 1:500 - 1:5,000
ELISA: 1:5,000 - 1:20,000
Reactivity Bacterial
Host Goat
Clonality Polyclonal
Immunogen N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
Specificity N-Acylmannosamine-1-Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified and partially purified N-Acylmannosamine-1-Dehydrogenase derived from Pseudomonas.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1 mg/mL - lot specific
Conjugation HRP
Storage Condition Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background N-Acylmannosamine-1-Dehydrogenase acts on acetyl-D-mannosamine and glycolyl-D-mannosamine. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Synonyms goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH
Note Anti-N-Acylmannosamine-1-Dehydrogenase has been assayed against 1.0 ug of N-Acylmannosamine-1-Dehydrogenase  IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 is suggested for this product.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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