glnA Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	WB: 1:500 - 1:2,500 ELISA: 1:2,000 - 1:10,000
Reactivity	Brevibacterium
Host	Goat
Clonality	Polyclonal
Immunogen	Glutamine Synthetase [native from brevibacterium species]
Specificity	Glutamine Synthetase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial].  Cross reactivity against Glutamine Synthetase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Database Link	UniProt ID Q79VE3
Background	Glutamine Synthetase catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. Glutamine Synthetase is present predominantly in the brain, kidneys, and liver. In the brain it participates in the metabolic regulation of glutamate, the detoxification of brain ammonia, the assimilation of ammonia, recyclization of neurotransmitters, and termination of neurotransmitter signals. Glutamine Synthetase, in the brain, is found primarily is astrocytes.
Synonyms	goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase
Note	Anti-Glutamine Synthetase has been assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.
Reference Data

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