glnA Goat Polyclonal Antibody
CAT#: TA396680S
Glutamine Synthetase Antibody Peroxidase Conjugated
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CNY 2,119.00
货期*
4周
规格
经常一起买 (1)
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommend Dilution | WB: 1:500 - 1:2,500 ELISA: 1:2,000 - 1:10,000 |
Reactivity | Brevibacterium |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Glutamine Synthetase [Microbial] |
Specificity | Glutamine Synthetase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Concentration | 1.0 mg/mL - lot specific |
Conjugation | HRP |
Storage Condition | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Database Link | |
Background | Glutamine Synthetase catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. Glutamine Synthetase is present predominantly in the brain, kidneys, and liver. In the brain it participates in the metabolic regulation of glutamate, the detoxification of brain ammonia, the assimilation of ammonia, recyclization of neurotransmitters, and termination of neurotransmitter signals. Glutamine Synthetase, in the brain, is found primarily is astrocytes. |
Synonyms | goat anti-Glutamine Synthetase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glutamine Synthetase Antibody, Glutamine synthetase |
Note | Anti-Glutamine Synthetase has been assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product. |
Reference Data |
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