Mouse Monoclonal Antibody [Clone ID: 4C7.D5]

Specifications

Product Data
Clone Name	4C7.D5
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 2 ug/mL FC: 1-2 µg/mL ELISA: 1:20,000
Host	Mouse
Clonality	Monoclonal
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Specificity	Biotin Antibody was prepared from mouse ascites by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.9mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store Biotin antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background	Localization of biotinylated probes with avidin produces may produce high background levels. Anti-biotin antibodies may be substituted for avidin to decrease non-specific binding.
Synonyms	mouse anti-Biotin Monoclonal Antibody, Anti-Biotin, Vitamin B4 antibody
Note	Anti-Biotin antibody has been tested in ELISA, western blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reference Data

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