Mouse Monoclonal Antibody [Clone ID: 4C7.D5]

CAT#: TA396747S

Biotin Monoclonal Antibody



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CNY 2,119.00


货期*
4周

规格
    • 25 ul

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Specifications

Product Data
Clone Name 4C7.D5
Applications ELISA, FC, IF, IHC, WB
Recommend Dilution WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 2 ug/mL
FC: 1-2 µg/mL
ELISA: 1:20,000
Host Mouse
Clonality Monoclonal
Immunogen Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Specificity Biotin Antibody was prepared from mouse ascites by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 0.9mg/mL - lot specific
Conjugation Unconjugated
Storage Condition Store Biotin antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background Localization of biotinylated probes with avidin produces may produce high background levels. Anti-biotin antibodies may be substituted for avidin to decrease non-specific binding.
Synonyms mouse anti-Biotin Monoclonal Antibody, Anti-Biotin, Vitamin B4 antibody
Note Anti-Biotin antibody has been tested in ELISA, western blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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