hemoglobin subunit gamma 1 (HBG1) Mouse Monoclonal Antibody [Clone ID: 4B3.B5.F3.B7]

Specifications

Product Data
Clone Name	4B3.B5.F3.B7
Applications	ELISA, WB
Recommend Dilution	WB: 1ug/mL ELISA: 1:20,000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH.
Specificity	This protein A purified mouse monoclonal antibody reacts specifically with human HbF gamma isoform. Anti-HbF is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, Hylobates lar, Macaca nemestrina, Macaca mulatta, Pongo pygmaeus, Pongo pygmaeus, Macaca fuscata fuscata, and Papio cynocephalus. This antibody does not react with the HbA, HbS, HbC, or HbA-2 isoforms.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	hemoglobin subunit gamma 1
Database Link	Entrez Gene 3047 Human UniProt ID P69891
Background	HbF antibodies detect the hemoglobin gamma isoform subunit. Functional alternate hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 gamma subunits (alpha-2 gamma-2). Hemoglobin F is elevated in newborns, reaching adult levels by 12 months. HbF levels are increased to as much as 5% to 10% in normal pregnancy. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Hemoglobin variants arise from mutations in the globin genes and sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. HbF antibody does not react other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.
Synonyms	mouse anti-HbF antibody, mouse anti-hemoglobin antibody, Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain, HBG1, HBG2, HbF Antibody, Sickle Cell Disease (SCD)
Note	Anti-Hemoglobin beta F (MOUSE) antibody has been tested ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.
Reference Data

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