MUC4 Mouse Monoclonal Antibody [Clone ID: 3C7.D8.E11.F5]

Specifications

Product Data
Clone Name	3C7.D8.E11.F5
Applications	ELISA, WB
Recommend Dilution	WB: 1:1000 ELISA: 1:000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This protein A purified antibody was prepared from tissue culture supernate produced by repeated immunizations with a peptide corresponding to an internal portion of human Mucin-4.
Specificity	This affinity-purified antibody is directed against human MUC4 protein. The product was purified from tissue culture supernate by protein A chromatography. A BLAST analysis was used to suggest cross reactivity with MUC4 from human based on 100% sequence homology with the immunogen. Reactivity with MUC4 from other sources is not known.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.99 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	mucin 4, cell surface associated
Database Link	Entrez Gene 4585 Human UniProt ID Q99102
Background	This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). MUC4 (Mucin 4, Cell Surface Associated) is a Protein Coding gene. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. Highly glycosylated proteins called mucins, are the major constituents of mucus; the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix. MUC4’s ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. MUC4 seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. These glycoproteins play an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 Tyr-1248. In polarized epithelial cells segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a co-receptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. May play a role in tumor progression. MUC4 is associated with diseases such as bile duct cancer, keratitis, adenosquamous carcinoma, and pancreatic adenocarcinomas. Anti-MUC4 Antibody is useful for researchers interested in digestion, cancer research, and extracellular matrix Antibodies.
Synonyms	Mouse Anti-MUC4 Antibody, Mouse Anti-Mucin 4 Antibody, MUC4, Mucin-4, Ascites sialoglycoprotein, ASGP, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, Mucin-4 alpha chain, Ascites sialoglycoprotein 1, ASGP-1, Mucin-4 beta chain, Ascites sialoglycoprotein 2, ASGP-2, MUC
Note	Anti-MUC4 Antibody was testing in ELISA, IHC, and Western Blot. Antibodies shows no cross reactivity to non-mucilated proteins. Positive control used in WB were HPAC, HPAF-II, and Capan-2 cell lines.
Reference Data

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