CRASP1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1,000 ELISA: 1:2,000
Reactivity	Borrelia burgdorferi
Host	Rabbit
Clonality	Polyclonal
Immunogen	MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-1 protein.
Specificity	This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi CRASP-1 protein. A BLAST analysis was used to suggest reactivity with CRASP-1 from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence. Partial cross-reactivity is expected against B. spielmanii, afzelii, and valaisiana sources based on 98% homology. Cross-reactivity with CRASP-1 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID Q66ZC1
Background	CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes.
Synonyms	rabbit anti-CRASP-1 Antibody, Borrelia burgdorferi CRASP-1, CRASP1, CRASP 1, Complement regulator acquiring protein 1
Note	This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 26.9 kDa in size corresponding to Borrelia burgdorferi CRASP-1 protein by Western blotting in the appropriate cell lysate or extract.
Reference Data

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