Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, WB
Recommend Dilution	WB: User Optimized IF: 1:50 - 1:250 FC: User Optimized FLISA: User Optimized
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Protein G (Streptococcus species)
Specificity	Anti-Protein G Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Protein G.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	FITC
Storage Condition	Store Protein G conjugated antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Background	Protein G is a protein that has the property of binging to immunoglobulins. It is a 65-kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.
Synonyms	rabbit anti-Protein G Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Protein G Antibody, Protein G FITC
Note	FITC Conjugated Anti-Protein G antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.