Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	ELISA: 1:2,000-1:20,000
Reactivity	Streptomyces avidinii
Host	Rabbit
Clonality	Polyclonal
Immunogen	Streptavidin (Streptomyces avidinii)
Specificity	Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background	Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10−14 mol/l to 10−15 mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin through the antibody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interaction.
Synonyms	rabbit anti-Streptavidin Antibody biotin Conjugation, biotin conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin BAC Antibody
Note	Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentration in immunoassays should be performed by the researcher.
Reference Data

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