Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommend Dilution	IF: 1:50-1:250 FC: 1:200-1:1,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Protein G  [Streptococcus species]
Specificity	Anti-Protein G Texas Red Conjugated Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein G  [Streptococcus species], anti-Rabbit IgG and anti-Rabbit Serum.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Texas Red
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background	Protein G is a protein that has the property of binging to immunoglobulins. It is a 65-kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.
Synonyms	rabbit anti-Protein G Antibody Texas Red™ Conjugation, Texas Red™ conjugated rabbit anti-Protein G Antibody, Protein G Texas Red™
Note	Anti-Protein G Texas Red is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.