ARG1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1,000 - 1:5,000 ELISA: 1:10,000 - 1:40,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Arginase [Bovine Liver]
Specificity	Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver].  Cross reactivity against Arginase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID Q2KJ64
Background	Arginase is a manganese-containing enzyme belonging to the ureohydrolase family of enzymes. Arginase catalyzes the fifth and final step in the urea cycle, a series of biochemical reactions in mammals during which the body disposes of harmful ammonia. Specifically, arginase converts L-arginine into L-ornithine and urea.
Synonyms	rabbit anti-Arginase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Arginase Antibody, A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody
Note	Anti-Arginase Peroxidase Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Arginase [Bovine Liver] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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