ADH1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	WB: 1:1,000 - 1:5,000 IHC: 1:500 - 1:2,500 ELISA: 1:4,000 - 1:20,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Alcohol Dehydrogenase [Yeast]
Specificity	Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].  Cross reactivity against Alcohol Dehydrogenase from other sources is unknown.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P00330
Background	Alcohol Dehydrogenases (ADH) are a group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In humans and many other animals, they serve to break down alcohols that otherwise are toxic, and they also participate in generation of useful aldehyde, ketone, or alcohol groups during biosynthesis of various metabolites.
Synonyms	rabbit anti-Alcohol Dehydrogenase Antibody Peroxidase Conjugation, HRP conjugated rabbit ant-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, ADH, ADC
Note	Anti-Alcohol Dehydrogenase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable in immunohistochemistry. Optimal titers are to be optimized by researchers.
Reference Data

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