XDH Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IP, WB
Recommend Dilution	WB: 1:250
Host	Rabbit
Clonality	Polyclonal
Immunogen	Xanthine Oxidase [Bovine Buttermilk]
Specificity	Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].  Cross reactivity against Xanthine Oxidase from other tissues and species may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P80457
Background	Xanthine Oxidase is a key enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine, xanthine to uric acid, and contributes to the generation of reactive oxygen species.
Synonyms	rabbit anti-Xanthine Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody
Note	Anti-Xanthine Oxidase Peroxidase conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Xanthine Oxidase [Bovine Buttermilk] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested for this product.
Reference Data

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