HXK2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 - 1:1,000 ELISA: 1:1,000 - 1:2,500
Host	Rabbit
Clonality	Polyclonal
Immunogen	Hexokinase [Yeast]
Specificity	Anti-Hexokinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other sources may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID P04806
Background	Anti-Hexokinase antibody recognizes hexokinase which phosphorylates hexoses. Hexokinase uses one ATP molecule when phosphorylating six-carbon sugars. In mammals there are four isozymes of hexokinase, the most notable of which is hexokinase IV, also known as glucokinase which is highly specific for glucose. Phosphorylation of hexoses commit them to certain metabolic pathways. For example, phosphorylation of glucose to glucose-6-phosphate limits it to glycolysis or glycogen synthesis. Anti-Hexokinase antibody is ideal for investigators interested in Metabolism, Cardiology, or Enzymology.
Synonyms	rabbit anti-Hexokinase Antibody Biotin Conjugation, Biotin Conjugated rabbit anti-Hexokinase Antibody, DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody
Note	Anti-Hexokinase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Hexokinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reference Data

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