pep1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:500 - 1:2,000 ELISA: 1:5,000 - 1:20,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phospho-enol-pyruvate Carboxylase [Maize Leaves]
Specificity	Anti-Phospho Enol Pyruvate Carboxylase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate-Carboxylase [Maize].  Cross reactivity against Phospho-enol-Pyruvate-Carboxylase from other sources may occur but have not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID B8XPZ2
Background	Anti-Phospho Enol Pyruvate Carboxylase antibody detects PEP. Phosphoenolpyruvate carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate. This reaction is used for carbon fixation in so-called "CAM" and "C4" plants where it plays a key role in photosynthesis. The enzyme is also found in some bacteria, but not in animals or fungi. Anti-Phospho Enol Pyruvate Carboxylase Antibody is ideal for investigators involved in Cell Signaling, biochemistry and Signal Transduction research.
Synonyms	rabbit anti-Phospho Enol Pyruvate Carboxylase Antibody biotin Conjugation, biotin conjugated rabbit anti-PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody
Note	Anti-Phospho Enol Pyruvate Carboxylase Biotin Conjugated antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Phospho-enol-Pyruvate-Carboxylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.
Reference Data

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