lacZ Chicken Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:2,000-1:10,000 ELISA: 1:2,000 - 1:10,000
Reactivity	Beta Galactosidase [E.coli]
Host	Chicken
Clonality	Polyclonal
Immunogen	This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli
Specificity	This Anti-Beta Galactosidase Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E.coli] and anti-Chicken Serum. Cross reactivity against Beta Galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	UniProt ID P00722
Background	Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.
Synonyms	chicken anti-Beta Galactosidase Antibody, chicken anti-Beta Gal Antibody, Beta Galactosidase [E.coli], BGAL, beta-galactosidase, beta-gal
Note	Anti-Beta-Gal (CHICKEN) Antibody has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity.
Reference Data

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