CXCL10 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1 µg/mL ELISA: 1:10,000
Reactivity	Goat
Host	Rabbit
Clonality	Polyclonal
Immunogen	This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 83 amino acids of the mature bovine CXCL10 protein.
Specificity	This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine CXCL10 protein. A BLAST analysis was used to suggest cross-reactivity with CXCL10 from bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against sheep and goat based on 94-95% homology. Cross-reactivity with CXCL10 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Database Link	Entrez Gene 615107 Bovine UniProt ID Q2KIQ8
Background	CXCL10 is a secreted chemotactic cytokine for monocytes and T-lymphocytes. It is involved in the inflammatory response. CXCL10 can be upregulated in response to infection. It binds to CXCR3.
Synonyms	rabbit anti-CXCL10 Antibody, IP-10, C-X-C motif chemokine 10, Small-inducible cytokine B10
Note	This protein-A purified CXCL10 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 9.3 kDa in size corresponding to bovine CXCL10 protein by western blotting in the appropriate cell lysate or extract.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.