Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: User Optimized IHC: User Optimized ELISA: User Optimized
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Rat Serum Proteins
Specificity	Anti-Rat serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background	Anti-Rat serum antibody detects rat serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Rat serum antibody is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.
Synonyms	rabbit anti-Rat Serum Antibody Peroxidase Conjugated, rabbit anti-Rat Serum HRP conjugated Antibody
Note	Anti-Rat serum Peroxidase Conjugated antibody has been tested by Dot blot and ELISA and is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.