Rabbit Polyclonal Antibody
CNY 2,119.00
货期*
4周
规格
经常一起买 (1)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
CNY 300.00
CNY 1,430.00
Specifications
Product Data | |
Applications | ELISA, FC, IF, IHC, IP, WB |
Recommend Dilution | WB: User Optimized IHC: 1:100-1:500 IF: 1:500 FC: 1:50-1:100 ELISA: 1:2000 - 1:10,000 |
Reactivity | BrdU |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH. |
Specificity | BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Concentration | 1.1 mg/mL - lot specific |
Conjugation | Unconjugated |
Storage Condition | Store BrdU Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Background | Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat. |
Synonyms | rabbit anti-BrdU antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine |
Note | Anti-BrdU Antibody has been tested by ELISA, WB, IHC, and is suitable for immunofluorescence microscopy and flow cytometry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated BrdU thymidine analog from replicated cells. |
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