Ccnd3 Rabbit Polyclonal Antibody

CAT#: TA397443S

Cyclin D3 Antibody

Size: 100 ug 25 ul



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CNY 2,119.00


货期*
4周

规格
    • 25 ul

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Specifications

Product Data
Applications ELISA, FC, IHC, IP, WB
Recommend Dilution WB: 1.0 ug/ml
IHC: 4µg/mL
FC: 4µg/mL
ELISA: 1:1000 - 1:5000
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
Specificity Cyclin D3 affinity-purified antibody is directed against Cyclin D3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Cyclin D3.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.0 mg/ml - lot specific
Conjugation Unconjugated
Storage Condition Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name cyclin D3
Background Anti-Cyclin D3 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Cyclin D3 belongs to a highly conserved cyclin family, whose members are the ultimate recipients of oncogenic signals. Cyclin D3 is a key component of the cell cycle progression machinery and induces progression through the G1 phase of the cell cycle. Cyclin D3 is expressed in nearly all proliferating cells, and shows the most broad expression pattern of all three D-type (D1-D3) cyclins. Cyclin D3 is encoded from the 6p21 chromosome region and the protein is predominantly localized in the nucleus. Once induced, cyclin D3 binds and activates its associated cyclin-dependent kinases CDK4 and CDK6. Amplification of the cyclin D3 gene and overexpression of cyclin D3 protein is seen in several human cancers. A large number of human malignancies contain lesions in pathways impacting on cyclin D3. Abnormal expression of Cyclin D3 is believed to be a driving force in several human cancers. A possible role for cyclin D3 in the malignancies of the lymphoid system is suggested by the observations that cyclin D3 gene is rearranged in several neoplastic diseases such as diffuse large B cell lymphomas or multiple myelomas. Anti-Cyclin D3 is ideal for researchers interested in Cancer Research and Immunology research.
Synonyms rabbit anti-Cyclin D3 antibody, G1/S-specific cyclin-D3, Ccnd3, Cyl-3
Note This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, flow cytometry, and by immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 32.4 kDa in size corresponding to Cyclin D3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. This reagent reacts equally to phosphorylated and non-phosphorylated Cyclin D3 by ELISA and western blotting.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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