H3C14 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, IF, IHC, WB
Recommend Dilution	WB: 1:500 IHC: 1:50 IF: 1:50 CHiP: 2-5µg/million cells
Reactivity	C. elegans, Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Histone H3 [Dimethyl Lys18] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 18 of human Histone H3.2.
Specificity	Anti-Histone H3 [Dimethyl Lys18] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.79 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Gene Name	histone cluster 2, H3c
Database Link	Entrez Gene 333932 HumanEntrez Gene 126961 Human UniProt ID Q71DI3
Background	The di-methylated K18 on histone H3 is a seemingly transient post-translational modification. H3K18 is better known to be acetylated, and occasionally mono-methylated. Suv39h1, a well-studied histone methyltransferase seems to be responsible for the transition of acetylation and methylation at this H3 modification site. The di-methylated K18 on H3 seems to be associated with embryological development and possibly implantation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
Synonyms	rabbit anti-Histone H3 dimethyl Lys18 antibody, H3.3AH3F3H3F3B, H3.3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K18me2
Note	Anti-Histone H3 [Dimethyl Lys18] antibody is tested in Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunofluorescence. This antibody is useful for Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Reference Data
Protein Pathways	Systemic lupus erythematosus

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