H3C15 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IP, WB
Recommend Dilution	WB: 1:1000 IF: 1:100 ELISA: 1: 10,000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Histone H3 [Trimethyl Lys27] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27me3 site of human Histone H3.
Specificity	Anti-Histone H3 [Trimethyl Lys27] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [Lys27 me3] from other sources has not been determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	0.93 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Database Link	UniProt ID Q71DI3
Background	The nucleosome is comprised of 146 bp of DNA wrapped around a series of histone proteins arranged as an octamer consisting of 2 copies of histone H2A, H2B, H3 and H4. Within the nucleosome core the histone proteins are covalent modified at specific residues predominantly within the N-terminal tail including lysine (acetylation, methylation, SUMOylation, and ubiquitinylation), arginine methylation and citrullination, serine and threonine phosphorylation, as well as proline isomerization. The lysine side chains can carry up to three methyl groups (mono-, di- and tri- methylated forms) and the arginine side chain can be monomethylated or can be dimethylated as the symmetric or asymmetric forms. The modifications show temporal, disease-specific, and other types of cell-specific regulation and there are specific families of enzymes that regulate the methylation, demethylation, acetylation, deacetylation and other modifications. Research has indicated that whereas the histone mark H3K4Me3 (tri-methyl lysine 4 of histone H3) localizes to gene promoter regions (it is associated with transcriptional activation) other modifications at H3K4 such as monomethyl is present predominantly at enhancer sequences. Specific marks have been shown to be associated with the activation (H3K9Me1, H3K27Me1, and H4K20Me1) or repression (H3K9Me2 and Me3, H3K27Me2 and Me3, and H4K20Me2 and Me3) of genes. Monomethylation of H4 at K20, catalyzed by SET8, is essential to genome replication and stability. Multiple DNA breaks are associated with demethylation at this site, resulting in activation of p53 to avoid mitosis and aberrant chromosomal activity. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones, and Phospho Specific research.
Synonyms	rabbit anti-Histone H3 trimethyl Lys27 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K27me3
Note	Anti-Histone H3 [Trimethyl Lys27] antibody is tested for Dot Blot, IF, and Western Blot. This antibody is useful for ELISA and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.