Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, WB
Recommend Dilution	WB: 1:2,000 - 1:10,000 IF: User Optimized FC: User Optimized ELISA: 1:60,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is full length GST isolated from Schistosoma japonicum.
Specificity	FITC conjugated GST Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration	1.0 mg/mL - lot specific
Conjugation	FITC
Storage Condition	Store FITC conjugated GST Antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Background	GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms	rabbit anti-GST antibody fluorescein conjugation, FITC conjugated rabbit anti-GST antibody, Glutathione-S-Transferase
Note	FITC conjugated GST Antibody has been tested by western blot and is ideal for microscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.