Collagen V (COL5A3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	WB: 1:5,000 to 1:10,000 IHC: 1:50 to 1:200 ELISA: 1:4,000 - 1:8,000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen Type V from human and bovine placenta
Specificity	Anti-Collagen V has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.  Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.   This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV and VI collagens.  Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Formulation	0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 50µL
Concentration	1.0 mg/mL - lot specific
Conjugation	HRP
Storage Condition	Store vial at 4° C prior to restoration.   Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.   Expiration date is one (1) year from date of restoration.
Gene Name	collagen type V alpha 3
Database Link	Entrez Gene 50509 Human UniProt ID P25940
Background	In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.
Synonyms	rabbit anti-Collagen Type V antibody peroxidase conjugation, HRP conjugated rabbit anti-Collagen Type V antibody, COL5A1 protein antibody, Collagen fetal membrane A polypeptide antibody, Collagen alpha-1 (V) chain
Note	Anti-Collagen V Peroxidase Conjugated Antibody was assayed by immunoblot and found to be reactive against Collagen V at a dilution of 1:5,000 to 1:10,000.  This product was also assayed against 1.0 ug of Collagen V in a standard sandwich ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product.  For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200.
Reference Data

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