rpmB Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 0.5 μg/mL. Antibody validated: Western Blot in E.coli samples. All other applications and species not yet tested.
Reactivity	Escherichia coli
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-rpmB/L28 antibody (10061) was raised against an 15 amino acid peptide near the amino terminus of E. coli rpmB/L28. The immunogen is located within the first 50 amino acids of rpmB/L28.
Formulation	rpmB/L28 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Purification	rpmB/L28 Antibody is affinity chromatography.
Conjugation	Unconjugated
Storage Condition	rpmB/L28 Antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Predicted Protein Size	Predicted: 9 kD Observed: 9 kD
Database Link	UniProt ID P0A7M2
Background	The rpmB/L28 protein is a component of the 50S subunit of the ribosome and is required for ribosome assembly. rpmB/L28 interacts with 23S rRNA and crosslinks to L9. L15 and L17 stimulate the interactions of rpmB/L28 with 23S rRNA. rpmB/L28 is phosphorylated at Thr8. rpmB/L28 is labeled by the macrolides carbomycin A, niddamycin and tylosin, which inhibit ribosomal activity.
Note	Optimal dilutions for each application to be determined by the researcher.
Reference Data

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