BOB1 (POU2AF1) Mouse Monoclonal Antibody [Clone ID: UMAB220]

Specifications

Product Data
Clone Name	UMAB220
Applications	10k-ChIP, IHC
Recommend Dilution	IHC 1:100
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human POU2AF1(NP_006226) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	27.3 kDa
Gene Name	POU class 2 homeobox associating factor 1
Database Link	NP_006226 Entrez Gene 18985 MouseEntrez Gene 690528 RatEntrez Gene 5450 Human UniProt ID Q16633
Background	Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. [UniProtKB/Swiss-Prot Function]
Synonyms	BOB1; OBF-1; OBF1; OCAB
Reference Data

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