MSH2 Mouse Monoclonal Antibody [Clone ID: UMAB259]

Specifications

Product Data
Clone Name	UMAB259
Applications	IHC
Recommend Dilution	IHC 1:2400
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-304 of human MSH2 (NP_000242) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	104.6 kDa
Gene Name	mutS homolog 2
Database Link	NP_000242 Entrez Gene 17685 MouseEntrez Gene 81709 RatEntrez Gene 4436 Human UniProt ID P43246
Background	This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Synonyms	COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; MMRCS2
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Colorectal cancer, Mismatch repair, Pathways in cancer

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