GPA33 Mouse Monoclonal Antibody [Clone ID: UMAB283]

Specifications

Product Data
Clone Name	UMAB283
Applications	IHC, WB
Recommend Dilution	WB 1:1000, IHC 1:400
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human GPA33 (NP_005805) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Predicted Protein Size	35.6 kDa
Gene Name	glycoprotein A33
Database Link	NP_005805 Entrez Gene 10223 Human UniProt ID Q99795
Background	The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008].
Synonyms	A33
Reference Data
Protein Families	Druggable Genome, Transmembrane

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