Duck IgM (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Duck IgM (Fc specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, WB
Recommend Dilution	Suitable for use in Immunocytochemical and iImunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. Immunohistochemistry and Immunocytochemistry: 1/50-1/250. Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivity	Duck
Host	Goat
Immunogen	Purified IgM isolated from Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized Hyperimmune IgG fraction Molar radio: Biotin/IgG ~ 6.3
Concentration	10 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and DEAE-Chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies crossreacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.