Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
CAT#: AP21490HR-N
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP
Conjugation: Biotin FITC TRITC
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CNY 7,710.00
Cited in 5 publications. |
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Specifications
Product Data | |
Product Name | Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody |
Applications | ELISA, ID, IF, IHC, IP, WB |
Recommend Dilution | ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids). Immunocytochemistry. Immunohistochemistry. Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties. Dot blot. Western blot. General Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen. |
Reactivity | Rabbit |
Host | Goat |
Immunogen | Purified immunoglobulin fractions isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Formulation | PBS, pH 7.2 without preservatives and foreign proteins Label: HRP State: Lyophilized purified IgG fraction Label: Horseradish Peroxidase Enzyme marker: Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2). Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life Molar radio: Peroxidase/IgG: ~1.7 |
Concentration | appr. 10.0 mg/ml |
Purification | Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt-Precipitation and purifaction of the IgG fraction by DEAE Chromatography |
Conjugation | HRP |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Citations (5)
The use of this Antibodies has been cited in the following citations: |
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Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice
,null,
Molecular Medicine Reports
,PubMed ID 33760102
|
Association between levels of aquaporin 3 in the placenta and adiponectin in the umbilical cord blood with gestational diabetes mellitus and pregnancy outcome
,null,
Molecular Medicine Reports
,PubMed ID 32627013
|
Receptor tyrosine kinase expression in high-grade gliomas before and after chemoradiotherapy
,null,
Oncology Letters
,PubMed ID 31807171
|
Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion
,null,
Oncology Letters
,PubMed ID 29844811
|
Preliminary study of the effect of abnormal savda munziq on TGF-β1 and Smad7 expression in hypertrophic scar fibroblasts
,null,
International Journal of Clinical and Experimental Medicine
,PubMed ID 25785025
|
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