Rat IgG2ab (subclass specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Rat IgG2ab (subclass specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, IP, WB
Recommend Dilution	Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000 - 1/10,000.
Reactivity	Rat
Host	Goat
Immunogen	Pools of purified homogenous IgG2a and IgG2b isolated from rat sera. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: HRP State: Lyphilized purified hyperimmune IgG fraction Label: Horseradish Peroxidase Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2) Conjugation procedure Conjugation is carried out using a proprietary modification of the periodate technique for the binding to peroxidase, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life Molar radio: Peroxidase/IgG ~1.0
Concentration	10,0 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.
Conjugation	HRP
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.