Mouse IgG3 (subclass specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgG3 (subclass specific) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, IP, WB
Recommend Dilution	This antibody can be used to identify and measure IgG3, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: 1/20-1/80.
Reactivity	Mouse
Host	Goat
Immunogen	Pools of purified homogenous IgG3 isolated from pooled Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: FITC State: Lyphilized purified hyperimmune IgG fraction Label: Fluorescein Isothiocyanate Isomer 1 Absorption emission: 492 nm / 515 nm Molar radio: Fluorochrome/IgG ~1.7
Concentration	lot specific
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation	FITC
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Note	Conjugation Procedure: A proprietary technique for the binding to FITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Adsorption: Undesired traces of antibody activity are eliminated by Iimmunoaffinity Chromatography as required.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.