Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
Applications	ELISA, ID, IF, IHC, IP, WB
Recommend Dilution	Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays between 1/2000 and 1/10000.
Reactivity	Monkey
Host	Goat
Immunogen	Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. Label: HRP State: Lyophilised hyperimmune Ig fraction Label: Horseradish Peroxidase Molar radio: 1,4
Concentration	10 mg/ml
Conjugation	HRP
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

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