Monkey IgG (H+L chain) Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Monkey IgG (H+L chain) Rabbit Polyclonal Antibody
Applications	ELISA, IF, IHC, IP
Recommend Dilution	Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Reactivity	Monkey
Host	Rabbit
Immunogen	Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 No preservative added. No foreign proteins added. Label: FITC State: Lyophilised hyperimmune Ig fraction Label: Fluorescein isothiocyanate isomer 1 Conjugation procedure: A proprietary technique for the binding to is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life Absorption emission: 492nm/515nm Molar radio: 1,4
Concentration	10 mg/ml
Purification	DEAE-column Chromatography
Conjugation	FITC
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.