Canine IgG (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Canine IgG (Fc specific) Goat Polyclonal Antibody
Applications	ID, IP
Recommend Dilution	Can be used as unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivity	Canine
Host	Goat
Immunogen	Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. State: Azide Free State: Lyophilised hyperimmune Ig fraction
Concentration	10 mg/ml
Purification	DEAE-column Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.