Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: AP33082BT-N

Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin

Conjugation: Biotin FITC HRP



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CNY 3,996.00


货期*
5周

规格
    • 1 ml

Product images

Specifications

Product Data
Product Name Sheep IgG (H+L chain) Rabbit Polyclonal Antibody
Applications ELISA, IF, IHC, WB
Recommend Dilution ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
Reactivity Sheep
Host Rabbit
Immunogen Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation PBS, pH 7.2
Label: Biotin
State: Lyophilized purified hyperimmune IgG fraction
Stabilizer: None
Preservative: None, as it may interfere with the antibody activity.
Label: N-Hydroxysuccinimidobiotin
Molar radio: Biotin/IgG ~4.2
Concentration 10.0 mg/ml
Purification Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation Biotin
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Note Adsorption: Not required
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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