Porcine IgG (H+L chain) Rabbit Polyclonal Antibody

Specifications

Product Data
Product Name	Porcine IgG (H+L chain) Rabbit Polyclonal Antibody
Applications	ELISA, IF, IHC
Recommend Dilution	ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry Frozen Sections. Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-colour staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80.
Reactivity	Porcine
Host	Rabbit
Immunogen	Purified normal IgG isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Formulation	PBS, pH 7.2 Label: TRITC State: Lyophilized purified hyperimmune IgG fraction Stabilizer: None Preservative: None, as it may interfere with the antibody activity. Label: Tetramethylrhodamine isothiocyanate isomer R Absorption emission: 554 nm / 573 nm
Concentration	10.0 mg/ml
Purification	Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation	TRITC
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Note	Adsorption: Not required. Fluorescent Marker: Tetramethylrhodamine isothiocyanate isomer R has an orange-red fluorescence. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell-counting procedures. Conjugation procedure: A proprietary technique for the binding to TRITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.