Goat IgG (H+L chain), adsorbed Donkey Polyclonal Antibody

Specifications

Product Data
Product Name	Goat IgG (H+L chain), adsorbed Donkey Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using Peroxidase conjugated strepavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product.
Reactivity	Goat
Host	Donkey
Immunogen	Goat IgG whole molecule.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as a preservative. Label: Biotin State: Lyophilized purified Ig fraction. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule.
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	Biotin
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.