Mouse IgG (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgG (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody
Recommend Dilution	Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/600 to 1/2,750 of the reconstitution concentration is suggested for this product.
Reactivity	Mouse
Host	Goat
Immunogen	Mouse IgG whole molecule.
Formulation	0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer. Label: AP State: Liquid (sterile filtered) F(ab')2 fragments. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	AP
Storage Condition	Store the antibody undiluted at 2-8°C DO NOT FREEZE. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

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