Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
Applications	ELISA, WB
Recommend Dilution	Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested for this product.
Reactivity	Mouse
Host	Goat
Immunogen	Mouse IgG F(c) fragment.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: Biotin State: Lyophilized F(ab')2 fragments Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Sodium Azide Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule.
Concentration	lot specific
Purification	Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.