Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody
Recommend Dilution	Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivity	Rat
Host	Goat
Immunogen	Rat IgG F(c) fragment
Formulation	0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.001 M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: AP State: Liquid (sterile filtered) F(ab')2 fragments. Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	AP
Storage Condition	The antibody is stable at 2-8°C as undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Dilute only prior to immediate use.

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