F(ab')2 Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Specifications

Product Data
Product Name	F(ab')2 Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:500-1:2,500 IHC: 1:200-1:1,000 ELISA: 1:2,000 - 1:10,000
Host	Goat
Immunogen	Rabbit IgG whole molecule
Formulation	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1.0 mg/mL - lot specific
Conjugation	Alkaline Phosphatase
Storage Condition	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Note	F(ab')2 Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

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