F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Specifications

Product Data
Product Name	F(Ab')2 Fragment Of Hamster IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:500-1:3000 IHC: 1:200-1:1000 ELISA: 1:2000 - 1:10,000
Host	Goat
Immunogen	Golden Syrian and Armenian Hamster IgG whole molecules
Formulation	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1 mg/mL - lot specific
Conjugation	Alkaline Phosphatase
Storage Condition	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Note	Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Golden Syrian and Armenian Hamster IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

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