Human GM-CSF ELISA Kit (48-well)
CNY 2,630.00
Specifications
Product Data | |
Description | Human GM-CSF ELISA Kit (48-well) |
Size | 1 x 48 wells |
Format | 48-well strip plate |
Assay Type | Solid Phase Sandwich ELISA |
Assay Length | 3 hours |
Signal | Colorimetric |
Curve Range | 31.25-2000pg/ml |
Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
Sample Volume | 20 uL |
Specificity | Natural and recombinant Human GM-CSF Ligand |
Sensitivity | 7pg/mL |
Reactivity | Human |
Interference | No significant interference observed with available related molecules. |
Components |
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Background | Granulocyte macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine with multiple effects on hematopoietic cells . It mobilizes CD34+ progenitor cells into the periphery and stimulates their proliferation, survival and differentiation into neutrophils,monocytes/ macrophages, eosinophils, and myeloid dendritic cells . On these terminallydifferentiated myeloid cells, GM-CSF is also needed for inducing their effector functions . In addition, GM-CSF has been shown to stimulate the proliferation and differentiation of the erythroid and megakaryoctye progenitor cells . GM-CSF is produced by a number of different cell types, including keratinocytes, mature and immature NK cells, type II alveolar cells), endothelial cells, monocytes, bone-marrow mesenchymal stem cells, CD4+ and CD8+ T cells, megakaryocytes, B cells, eosinophils, chondrocytes and fibroblasts. Human GM-CSF cDNA encodes a 144 amino acid (aa) residue precursor protein with a 17 aa putative signal peptide and a 127 aa mature protien . Natural GM-CSF is a monomer that contains both N- and O-linked glycosylation . Mature human GM-CSF shares approximately 55%, 63% and 68% aa sequence homology with mouse, rat and canine. GM-CSF, respectively. Human GM-CSF is not biologically active on mouse cells , but was reported to have some activity on canine cells . GM-CSF exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins. The presence of the spliced variants in the heteromeric receptor complex can regulate the functions of the complex . |
Gene Symbol | CSF2 |
Standard Curve |
![]() Representative standard curve for GM-CSF ELISA. GM-CSF was diluted in serial two-fold steps in Sample Diluent.
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Documents
Product Manuals |