Mouse VEGF ELISA Kit (96-well)

Specifications

Product Data
Description	Mouse VEGF ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hour
Signal	Colorimetric
Curve Range	0.02 - 1.0 ng/mL
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Mouse VEGF Ligand
Sensitivity	7pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to mouse VEGF (8 x 12) 2 vials mouse VEGF Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-mouse VEGF antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adults . It is a member of the plateletderived growth factor family that is characterized by a cysteine knot structure formed by eight conserved cysteine residues . Mouse embryos expressing only the VEGF120 isoform do not survive to term and show defects in skeletogenesis . Mouse VEGF120 shares 98% aa sequence identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt 1) and VEGF R2 (Flk1/KDR) on endothelial cells . VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells . In adults, VEGF functions mainly in wound healing and the female reproductive cycle . Pathologically, it is involved in tumor angiogenesis and vascular leakage . Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus .VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-. .
Gene Symbol	Vegfa
Standard Curve	Representative standard curve for VEGF ELISA. VEGF was diluted in serial two-fold steps in Sample Diluent.

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