Human HGF ELISA Kit

Specifications

Product Data
Description	Human HGF ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human HGF
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	125pg/ml-8000pg/ml
Sample Type	Cell culture supernates, serum and plasma(EDTA, citrate).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human HGF
Sensitivity	<5pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human HGF antibody 1 Lyophilized recombinant human HGF standard 10ng/tubex2 Biotinylated anti- human HGF antibody 100ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 100ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Plate Sealers 4 x Wash Buffer (25x) 20 ml
Background	Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
Gene Symbol	HGF
Standard Curve	Representative standard curve for Human HGF PicoKine ELISA Kit

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