Rat Neurotrophin-3 ELISA Kit

Specifications

Product Data
Description	Rat Neurotrophin-3 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Rat Neurotrophin-3
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	15.6pg/ml-1000pg/ml
Sample Type	Cell culture supernates and serum.
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of rat Neurotrophin-3
Sensitivity	<2pg/ml
Reactivity	Rat
Cross Reactivity	There is cross-reactivates with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- rat Neurotrophin-3 antibody 1 Lyophilized recombinant rat Neurotrophin-3 standard 10ng/tubex2 Biotinylated anti- rat Neurotrophin-3 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml
Background	Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
Gene Symbol	Ntf3
Standard Curve	Representative standard curve for Rat Neurotrophin-3 PicoKine ELISA Kit

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