Human P-Selectin ELISA Kit

Specifications

Product Data
Description	Human P-Selectin ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human P-Selectin
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	156 pg/ml-10.000 pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human P-Selectin
Sensitivity	<5pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant cross-reactivity or interference between SELP and its analogs was observed.
Components	96-well plate precoated with anti-human P-Selectin antibody | 1 Lyophilized recombinant human P-Selectin antibody standard | 10ng/tube x 2 Biotinylated anti-human P-Selectin antibody antibody | 100 ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100) Sample diluent buffer | 30ml Antibody diluent buffer | 12ml ABC diluent buffer | 12ml TMB color developing agent | 10ml TMB stop solution | 10ml Plate Sealers | 4 Wash Buffer (25x) | 20 ml
Background	P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.
Gene Symbol	SELP
Standard Curve	Representative standard curve for Human P-Selectin PicoKine ELISA Kit

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